Elie Saliba received his PhD diploma

Elie thesis Dec 2017
On December 20th, Elie Saliba publicly presented his PhD work dealing with a novel mechanism of activation of the TORC1 kinase complex in yeast. He has successfully received his PhD diploma. A great moment for Elie, who was surrounded by his wife, colleagues, and friends. Elie will stay in the lab for a postdoc where he will pursue his work about the role of H+ ATPases in activation of TORC1. Thanks a lot to him for his key contribution to this novel research topic of the lab.  

Georgios Kapetanakis obtained a FRIA fellowship

photo George Kapetanakis
Georgios Kapetanakis was informed by the FNRS that he’s among the laureates of the 2017 competition for FRIA fellowships. Georgios obtained his master diploma last year in the University of Athens (Greece). He did a master thesis work in the lab of Pr. G. Diallinas where he investigated the role of membrane transporters in resistance to specific drugs by the fungus Aspergillus nidulans. During his PhD, Georgios Kapetanakis will investigate the molecular mechanisms of the still-poorly understood phenomenon of amino acid excretion by yeast, in the context of a collaboration with Dr. Isabelle Georis of the IRMW institute (Anderlecht).  

ASCB 2017 meeting in Philadelphia

B André attended the annual meeting of the American Society of Cell Biology (ASCB) in Philadelphia (2-6 December). The EMBO was a coorganizer of this major annual cell biology meeting.   

Charlotte Felten hired by the lab as technician

We are pleased to announce that the lab has hired Charlotte FELTEN as a new technician. Charlotte obtained her diploma in 2017 at the “Haute Ecole de Louvain en Hainaut” (Fleurus). She did an internship in the Immunobiology group of our institute. Charlotte now joined the lab thanks to a new four-year grant from the Wallonie Region.

Study on FTY720 anticancer drug provoking endocytosis of amino acid transporters in yeast and human cells published in Scientific Reports

FTY720 is a drug causing death of several types of tumour cells. This effect is largely due to its ability to provoke endocytosis of several nutrient transporters. FTY720 also promotes endocytosis of amino acid permeases in yeast. In a study now published in Scientific Reports, Céline Barthelemy and coll. report that FTY720 inhibits the intrinsic activity of  transporters, in both yeast and human cells. This is coupled to rapid inhibition of the TORC1 kinase complex, in turn promoting endocytosis of the inactivated transporters. This study sheds new light on how FTY720 kills cancer cells.       

“Endocytic trafficking and signaling in health and disease” meeting

Céline Barthelemy attended the EMBO conference presenting the last research progress about “Endocytic trafficking and signaling in health and disease” that was held in Serock, Poland (10-15 September). Céline presented in a poster her last results about the mechanisms through which the anticancer drug FTY720 provokes endocytosis of amino acid transporters in yeast and human cells.    

Yeast Genetics and Molecular Biology meeting (Prague, August 2017)

Melody Cools, Elie Saliba and Bruno André have attended the last Yeast Genetics and Molecular Biology meeting that took place in Prague (Czech Republic) between August 27 and September 1. The opening lecture was given by Pr. Yoshinori Ohsumi (Nobel Prize in Physiology or Medicine 2016) about the “Molecular mechanism and physiological role of autophagy”. Melody and Elie were invited to give oral presentations in workshops organized during the meeting. The conference was a great opportunity to learn many novel things, and share and discuss data with colleagues.  

Elucitated mechanism of Can1 transporter ubiquitylation published in MBoC


Our last paper entitled “Transition of yeast Can1 transporter to the inward-facing state unveils an alpha-arrestin target sequence promoting its ubiquitylation and endocytosis” has just been published in the journal Molecular Biology of the Cell (MBoC). This study reports that ubiquitylation and endocytosis of the yeast Can1 transporter are triggered by a substrate-elicited change of its conformation (shift to the inward-facing state) coupled to activation of the TORC1 kinase complex. The experimental work was mainly conducted by Christos Gournas and the data of Can1 structural modeling were generated by Martine Prévost and Maria-Eva Krammer (ULB, Structure and Function of Biological Membranes). 

Minos Evangelinos obtained an FNRS postodc grant for joining the lab

We are very glad to welcome Dr Minos Evangelinos as a new FNRS postdoc (“Chargé de recherche”) in the lab. Minos obtained his PhD this year, after a research in the excellent lab of Pr. George Diallinas (University of Athens, Greece). Among other contributions, Minos reported an interesting case of degradation by selective autophagy of a membrane transporter stacked in the endoplasmic reticulum in the model fungus Aspergillus nidulans (Evangelinos et al. 2016). He also set up a novel mutagenesis tool based on a transposon (named .. Minos) for functional genomic analysis in A. nidulans (Evangelinos et al. 2014). Welcome to him ! 

A visit of Kassem Ghaddar to the lab

KassemJune2017 - 1
Kassem GHADDAR with Catherine JAUNIAUX

Kassem Ghaddar very recently made a surprise visit to the lab. It was so nice to see him again, after such a long time 🙂  

Since October 2016, Kassem is Clinical Research Associate at the INC company. Before this, he did his master at ULB (2006-2009), a PhD in our lab thanks to a FRIA fellowship (2010-2014), and then a postdoc in the lab of Miriam Cnop and Decio Eizirik (ULB, Center for Diabetes Research (2014-2016).  It was great to see him very happy in his new job, and we of course remembered the great moments he spent in the lab.

During his PhD work, Kassem buit the first 3D models of the yeast Can1 arginine transporter in collaboration with Martine Prevost and Eva-Maria Krammer (ULB), and succeeded in converting the protein into a lysine transporter (Ghaddar et al. 2014a). He then found that the conformational changes undergone by the protein during catalysis of transport promotes its ubiquitylation and endocytosis (Ghaddar et al. 2014b).