Milligrams of Gap1 purified

In a novel paper by Debailleul et al. published in Microbial Cell Factories in collaboration with Dr. Cédric Govaerts (Lab of Structure and function of biological membranes, ULB), we report the development of a new inductible expression system in yeast based on the promoter of the GAP1 gene encoding the general amino acid permease. Using this system, a protocol has been implemented to purify in a few days milligrams of the Gap1 permease from yeast cells. This opens important perspectives for future works on the structure-function relationships of this membrane transporter.

Nitrogen catabolite repressible GAP1 promoter, a new tool for efficient recombinant protein production in S. cerevisiaeDebailleul F, Trubbia C, Frederickx N, Lauwers E, Merhi A, Ruysschaert JM, André B, Govaerts C.
Microb Cell Fact. 2013 Dec 26;12(1):129.

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